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Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons

DOI :

10.3791/51139-v

February 12th, 2014

February 12th, 2014

15,140 Views

1Department of Anatomy & Neuroscience, The University of Melbourne, 2Department of Neurobiology, Duke University Medical Center, 3Florey Institute of Neuroscience & Mental Health, The University of Melbourne

We describe a method to label protein on the surface of living neurons using a specific polyclonal antibody to extracellular epitopes. Protein bound by the antibody on the cell surface and subsequently internalized via endocytosis can be distinguished from protein remaining on, or trafficked to, the surface during the incubation.

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