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An Engulfment Assay: A Protocol to Assess Interactions Between CNS Phagocytes and Neurons

DOI :

10.3791/51482-v

June 8th, 2014

June 8th, 2014

17,762 Views

1Department of Neurology, F.M. Kirby Neurobiology Center, Boston Children's Hospital, Harvard Medical School

Microglia are the resident immune cells of the central nervous system (CNS) with a high capacity to phagocytose or engulf material in their extracellular environment. Here, a broadly applicable, reliable, and highly quantitative assay for visualizing and measuring microglia-mediated engulfment of synaptic components is described.

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Keywords Phagocytosis

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