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DOI :
10.3791/51293-v
March 26th, 2014
Chapters
0:05
Title
1:31
Plant Growth and Inoculation with Agrobacterium
3:09
Preparation of Cell-free Extracts
4:08
Detecting Protein Degradation by Immunoblotting
6:01
Results: VBF Promotes Proteasomal Degradation of VIP1
7:07
Conclusion
標的タンパク質分解は、細胞機能の主要な調節機構を表す。その後、26Sプロテアソームのために、分子「タグ」を提供する標的タンパク質にユビキチン鎖が付加し保存され、ユビキチン-プロテアソーム経路を介して行われます。ここでは、タンパク質のプロテアソーム分解のための、シンプルで信頼性の高い無細胞アッセイを記述します。
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