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Endothelial Cell Tube Formation Assay for the In Vitro Study of Angiogenesis

DOI :

10.3791/51312-v

September 1st, 2014

September 1st, 2014

57,409 Views

1Department of Biology, American University, 2Laboratory of Cancer Biology and Genetics, National Cancer Institute, NIH

The tube formation assay is a fast, quantifiable method for measuring in vitro angiogenesis. Endothelial cells are combined with conditioned media and plated on basement membrane extract. Tube formation occurs within hours and newly formed tubules easily quantified.

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Endothelial Cell Tube Formation Assay

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