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Three-dimensional Angiogenesis Assay System using Co-culture Spheroids Formed by Endothelial Colony Forming Cells and Mesenchymal Stem Cells

9.8K Views

09:24 min

September 18th, 2019

DOI :

10.3791/60032-v

September 18th, 2019


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Angiogenesis

Chapters in this video

0:04

Title

1:06

Endothelial Colony Forming Cell (ECFC) and Mesenchymal Stem Cell (MSC) Suspension Preparation and Fluorescent Dye Labeling

3:20

ECFC, MSC, or ECFC+MSC Spheroid Generation

4:12

Neutralized Collagen Gel Spheroid Embedding and Spheroid Sprouting Quantification

6:29

Results: Representative Sprout Formation from ECFC-only, MSC-only, and ECFC+MSC Spheroids

8:44

Conclusion

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