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Using an α-Bungarotoxin Binding Site Tag to Study GABA A Receptor Membrane Localization and Trafficking

DOI :

10.3791/51365-v

March 28th, 2014

March 28th, 2014

15,596 Views

1Pharmacology & Chemical Biology Department, University of Pittsburgh School of Medicine

Here we demonstrate the use of fluorescent Alexa dye coupled to α-bungarotoxin to measure GABA A receptor surface localization and endocytosis in hippocampal neurons. Through the use of constructs bearing a short extracellular tag that binds α-bungarotoxin, analysis of plasma membrane protein endocytic trafficking can be achieved.

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Bungarotoxin

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