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High-Resolution Quantitative Immunogold Analysis of Membrane Receptors at Retinal Ribbon Synapses

DOI :

10.3791/53547-v

February 18th, 2016

February 18th, 2016

7,298 Views

1Synaptic Physiology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 2Advanced Imaging Core, National Institute on Deafness and Other Communication Disorders, National Institutes of Health

The postembedding immunogold method is one of the most effective ways to provide high-resolution analyses of the subcellular localization of specific molecules. Here we describe a protocol to quantitatively analyze glutamate receptors at retinal ribbon synapses.

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High Resolution

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