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Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds

DOI :

10.3791/51411-v

March 27th, 2014

March 27th, 2014

65,965 Views

1Forage-Animal Production Research Unit, Agricultural Research Service, United States Department of Agriculture

Methods are described for thin-layer chromatographic (TLC) separation of plant extracts and contact bioautography to identify antibacterial metabolites. The methods are applied to the screening of red clover phenolic compounds inhibiting hyper ammonia-producing bacteria (HAB) native to the bovine rumen.

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Thin layer Chromatographic TLC Separations

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