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Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA

DOI :

10.3791/51543-v

June 25th, 2014

June 25th, 2014

29,370 Views

1Department of Translational Oncology, National Center for Tumor Diseases (NCT) and German Cancer Research Center (DKFZ)

Linear-amplification mediated (LAM)-PCR is a method developed to identify the exact positions of integrating viral vectors in the genome. The technique has evolved to be the superior method to study clonal dynamics in gene therapy patients, biosafety of novel vector technologies, T-cell diversity, cancer stem cell models, etc.

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