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Characterization of MLKL-mediated Plasma Membrane Rupture in Necroptosis

DOI :

10.3791/58088-v

August 7th, 2018

August 7th, 2018

10,493 Views

1Department of Structural Biology, St. Jude Children's Research Hospital, 2Department of Chemical Biology and Therapeutics, St. Jude Children's Research Hospital, 3Department of Immunology, St. Jude Children's Research Hospital

We report methods for characterization of MLKL-mediated plasma membrane rupture in necroptosis including conventional and confocal live-cell microscopy imaging, scanning electron microscopy, and NMR-based lipid binding.

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