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Quantifying Single Microvessel Permeability in Isolated Blood-perfused Rat Lung Preparation

DOI :

10.3791/51552-v

June 30th, 2014

June 30th, 2014

9,302 Views

1Department of Physiology, The University of Tennessee Health Science Center, 2Department of Orthopedic Surgery and Biomedical Engineering, The University of Tennessee Health Science Center

The isolated blood-perfused lung preparation makes it feasible to visualize microvessel networks on the lung surface. Here we describe an approach to quantify permeability of single microvessels in isolated lungs using real time fluorescence imaging.

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Keywords Single Microvessel Permeability

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