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In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy

DOI :

10.3791/51669-v

August 6th, 2014

August 6th, 2014

13,016 Views

1Light Microscopy Core Facility, NHLBI/NIH, 2Hematology Branch, NHLBI/NIH

Combinatorial 5 fluorescent proteins marking of hematopoietic stem and progenitor cells allows in vivo clonal tracking via confocal and two-photon microscopy, providing insights into bone marrow hematopoietic architecture during regeneration. This method allows non-invasive fate mapping of spectrally-coded HSPCs-derived cells in intact tissues for extensive periods of time following transplantation.

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In Vivo Clonal Tracking

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