September 4th, 2014
•The nature of the interactions between hematopoietic stem and progenitor cells (HSPCs) and bone marrow niches is poorly understood. Custom hardware modifications and a multi-step acquisition protocol allow the use of two-photon and confocal microscopy to image ex vivo labeled HSPCs homed within bone marrow areas, tracking interactions and movement.
Tags
Related Videos
Transplantation of Whole Kidney Marrow in Adult Zebrafish
Homing of Hematopoietic Cells to the Bone Marrow
Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells
Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors
In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy
Osteoclast Derivation from Mouse Bone Marrow
A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions
Improving the Accuracy of Flow Cytometric Assessment of Mitochondrial Membrane Potential in Hematopoietic Stem and Progenitor Cells Through the Inhibition of Efflux Pumps
In Vivo Two-photon Imaging of Megakaryocytes and Proplatelets in the Mouse Skull Bone Marrow
Assessment of Cellular Bioenergetics in Mouse Hematopoietic Stem and Primitive Progenitor Cells using the Extracellular Flux Analyzer
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved