Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry
12.0K Views
•
07:57 min
•
December 26th, 2019
DOI :
December 26th, 2019
•Mukul Girotra*1,2, Anne-Christine Thierry3, Alexandre Harari1,3, George Coukos1, Olaia Naveiras2,4, Nicola Vannini*1
1Department of Oncology UNIL CHUV, Ludwig Institute for Cancer Research Lausanne, University of Lausanne, 2Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology Lausanne (EPFL), 3Center of Experimental Therapeutics, Department of Oncology, Centre Hospitalier Universitaire Vaudois, 4Hematology Service, Department of Oncology, Centre Hospitalier Universitaire Vaudois (CHUV)
Chapters in this video
0:04
Title
1:59
Ex Vivo Culture of FACS Sorted Hematopoietic Stem Cells
4:00
Measurement of Mitochondrial Mass and Membrane Potential
6:21
Results: Mitochondrial Profiles
7:18
Conclusion
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