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DOI :
10.3791/51850-v
•
11:08 min
October 16th, 2014
Chapters
0:05
Title
1:57
Determination of Mutation Rate and Frequency
2:50
Biotin Labeling
4:44
Bead Labeling and Magnetic Sorting
7:25
Determination of Replicative Age
9:09
Results: Mutation Frequencies and Rates in Yeast
10:39
Conclusion
突变率在通过波动测试测量年轻酿酒酵母细胞被用于预测不同的复制年龄的母细胞的突变频率。磁分选和流式细胞术,然后用于测量实际的突变频率和母细胞的年龄,以确定从预测的突变频率的任何偏差。
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