JoVE Logo
Faculty Resource Center

Sign In

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging

DOI :

10.3791/52136-v

January 5th, 2015

January 5th, 2015

15,954 Views

1Experimental Radiology, Institute of Diagnostic and Interventional Radiology I, Jena University Hospital, 2Department of Pharmaceutical Technology, Friedrich-Schiller-University Jena, 3Center for Electron Microscopy, Jena University Hospital

The use of fluorophores for in vivo imaging can be greatly limited by opsonization, rapid clearance, low detection sensitivity and cytotoxic effects on the host. Encapsulation of fluorophores in liposomes by film hydration and extrusion leads to fluorescence quenching and protection which enables in vivo imaging with high detection sensitivity.

Tags

Fluorescence quenching

-- Views

Related Videos

article

Three-dimensional Optical-resolution Photoacoustic Microscopy

article

A Cre-Lox P Recombination Approach for the Detection of Cell Fusion In Vivo

article

Viral Nanoparticles for In vivo Tumor Imaging

article

Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides

article

Selective Cell Elimination from Mixed 3D Culture Using a Near Infrared Photoimmunotherapy Technique

article

Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo

article

The Synthesis of RGD-functionalized Hydrogels as a Tool for Therapeutic Applications

article

Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier

article

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers

article

A Novel Surgical Technique As a Foundation for In Vivo Partial Liver Engineering in Rat

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved