March 7th, 2015
•Mature adipocytes may represent an abundant source of stem cells through dedifferentiation, which leads to a homogenous population of fibroblast-like cells. Collagenase digestion is used to isolate mature adipocytes from human fat. The goal of our protocol is to obtain multipotent, dedifferentiated fat cells from human mature adipocytes.
Tags
Related Videos
Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes
Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions
Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis
Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
Generation of Induced Pluripotent Stem Cells from Human Melanoma Tumor-infiltrating Lymphocytes
Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors
Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells
Hypoxic Preconditioning of Marrow-derived Progenitor Cells As a Source for the Generation of Mature Schwann Cells
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved