A Human 3D Extracellular Matrix-Adipocyte Culture Model for Studying Matrix-Cell Metabolic Crosstalk
9.2K Views
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09:04 min
•
November 7th, 2019
DOI :
November 7th, 2019
•Carmen G. Flesher1, Nicki A. Baker1, Clarissa Strieder-Barboza1,2, Dominic Polsinelli5, Phillip J. Webster1,2, Oliver A. Varban1, Carey N. Lumeng2,3,4, Robert W. O'Rourke1,6
1Department of Surgery, University of Michigan Medical School, 2Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, 3Graduate Program in Immunology, University of Michigan Medical School, 4Graduate Program in Cellular and Molecular Biology, University of Michigan Medical School, 5Undergraduate Research Opportunity Program, University of Michigan, 6Department of Surgery, Ann Arbor Veterans Affairs Healthcare System
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Chapters in this video
0:05
Title
1:20
Adipose Tissue Procurement and Preadipocyte Isolation
2:51
Adipose Tissue ECM Preparation
5:08
ECM-Adipocyte Preparation
7:04
Results: Genotypic and Phenotypic Characterization of In vitro Differentiated Adipocytes
8:36
Conclusion
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