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DOI :
10.3791/52505-v
January 26th, 2015
Chapters
0:05
Title
2:37
Transfection of Rat Basophilic Leukemia (RBL) Cells
6:06
Measuring NPY mRFP Exocytosis
7:48
Time-lapse Microscopy of Exocytosis
10:51
Image Analyses
13:00
Results: Quantifying Mast Cells Secretory Granule (SG) Size and their Exocytosis
14:44
Conclusion
本プロトコルの目的は、マスト細胞の分泌の機能的ゲノム解析を可能にする方法を開発することであった。プロトコルは、目的の遺伝子とcotrasfected蛍光レポーター遺伝子の放出を定量的に評価し、実時間に基づいて分泌顆粒の形態の分析。
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