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Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

DOI :

10.3791/52586-v

10:28 min

April 14th, 2015

April 14th, 2015

32,485 Views

1Diabetes and Islet Biology Group, NHMRC Clinical Trials Centre, Faculty of Medicine, The University of Sydney, 2Biomarkers Laboratory, NHMRC Clinical Trials Centre, Faculty of Medicine, The University of Sydney

Circulating microRNAs have recently emerged as promising and novel biomarkers for various cancers and other diseases. The goal of this article is to discuss three different probe-based real-time PCR platforms and methods that are available to quantify and determine the abundance of circulating microRNAs.

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Probe based Real time PCR

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