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11.4K Views
•
09:03 min
May 29th, 2014
DOI :
10.3791/51408-v
Chapters
0:05
Title
1:20
Preparing hESCs for FACS Purification
2:26
Lysis of FACS-purified Single Cell in Each Well of a 96-well Plate
3:28
Reverse Transcription
4:29
Amplification
5:26
qRT-PCR Performance
6:21
Results: qRT-PCR is an Efficient Method for Profiling Single Cell Gene Expression
8:39
Conclusion
Summary
Single cell gene expression assay is needed for understanding stem cell heterogeneities.
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