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qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

DOI :

10.3791/52941-v

7:00 min

May 25th, 2015

May 25th, 2015

16,125 Views

1Department of Biochemistry and Molecular Pharmacology, Institute for Systems Genetics, 2Roche Life Science, USA

Designer chromosomes of the Synthetic Yeast Genome project, Sc2.0, can be distinguished from their native counterparts using a PCR-based genotyping assay called PCRTagging, which has a presence/absence endpoint. Here we describe a high-throughput real time PCR detection method for PCRTag genotyping.

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QPCRTag Analysis

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