Sign In

JoVE Journal

Immunology and Infection

A subscription to JoVE is required to view this content.

Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells

8.9K Views

09:26 min

February 20th, 2015

February 20th, 2015

8,859 Views

0:05

Title

1:28

Stimulation and Collection of Microparticles

3:26

Characterization of Microparticles via FACS Analysis

5:10

Preparation of Bronchial Tissue Explants

6:44

Lymphocytic Microparticle Treatment

7:51

Results: Lymphocytic Microparticle Detection and Analysis

8:57

Conclusion

Cell membrane–shed microparticles (MPs) are active biological vesicles that can be isolated and their pathophysiological effects investigated in various models. Here we describe a method for generating MPs derived from T lymphocytes (LMPs) and for demonstrating their proapoptotic effect on airway epithelial cells.

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More