September 19th, 2013
•This protocol details a method to isolate antigen presenting cells from human thymus via different steps of enzymatic digestion of the tissue followed by density centrifugation of the single cell suspension and finally magnetic and/or FACS sorting of the cell populations of interest.
Tags
Related Videos
Isolation of Mouse Lung Dendritic Cells
Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine
Isolation, Identification, and Purification of Murine Thymic Epithelial Cells
T Cells Capture Bacteria by Transinfection from Dendritic Cells
Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols
Generation of Human Monocyte-derived Dendritic Cells from Whole Blood
Isolation of Dendritic Cells from the Human Female Reproductive Tract for Phenotypical and Functional Studies
Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α
Isolation and Adoptive Transfer of High Salt Treated Antigen-presenting Dendritic Cells
Isolation of Salivary Epithelial Cells from Human Salivary Glands for In Vitro Growth as Salispheres or Monolayers
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved