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Layer-by-layer Collagen Deposition in Microfluidic Devices for Microtissue Stabilization

DOI :

10.3791/53078-v

September 29th, 2015

September 29th, 2015

9,208 Views

1Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, 2Shriners Hospitals for Children-Boston

The creation of functional microtissues within microfluidic devices requires the stabilization of cell phenotypes by adapting traditional cell culture techniques to the limited spatial dimensions in microdevices. Modification of collagen allows the layer-by-layer deposition of ultrathin collagen assemblies that can stabilize primary cells, such as hepatocytes, as microfluidic tissue models.

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Microfluidic Devices

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