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Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex

DOI :

10.3791/53528-v

March 13th, 2016

March 13th, 2016

11,227 Views

1Department of Molecular and Cellular Neuroscience, Nencki Institute of Experimental Biology, 2Department of Biochemistry, Nencki Institute of Experimental Biology, 3Neurobiology Centre, Nencki Institute of Experimental Biology

This video shows the craniotomy procedure that allows chronic imaging of neurons in mouse retrosplenial cortex using in vivo two photon microscopy in Thy1-GFP transgenic line. This approach is combined with injection of mCherry-expressing adeno-associated virus into dorsal hippocampus. These techniques allow long-term monitoring of experience-dependent structural plasticity in RSC.

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Two photon Imaging

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