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Two-Photon in vivo Imaging of Dendritic Spines in the Mouse Cortex Using a Thinned-skull Preparation

DOI :

10.3791/51520-v

May 12th, 2014

May 12th, 2014

17,535 Views

1Department of Molecular Cell and Developmental Biology, University of California, Santa Cruz

Time-lapse imaging in the living animal provides valuable information on structural reorganization in the intact brain. Here, we introduce a thinned-skull preparation that allows transcranial imaging of fluorescently labeled synaptic structures in the living mouse cortex by two-photon microscopy.

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Two Photon Imaging

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