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Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain

DOI :

10.3791/53658-v

12:14 min

February 12th, 2016

February 12th, 2016

33,605 Views

1Ischemia Research Unit, Fraunhofer Institute for Cell Therapy and Immunology, 2Fraunhofer Research Institution for Marine Biotechnology, University of Lubeck, 3Department of Neurology, University of Leipzig

Inflammation plays a central role in the pathogenesis of ischemic stroke. Increasing evidence suggests that it acts as a double-edged sword which exacerbates early brain injury, but also contributes to later repair. This protocol describes the isolation of immune cells from the ischemic brain and their subsequent flow cytometric phenotyping.

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Isolation

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