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Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

DOI :

10.3791/53676-v

November 28th, 2015

November 28th, 2015

15,435 Views

1Department of Neurosurgery, University of Michigan, 2Department of Cell and Developmental Biology, University of Michigan

Presented here is a straightforward method for the isolation and flow cytometric analysis of glioma-infiltrating peripheral blood mononuclear cells that yields time-dependent quantitative data on the number and activation status of immune cells entering the early brain tumor microenvironment.

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Isolation

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