JoVE Logo
Faculty Resource Center

Sign In

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

DOI :

10.3791/53837-v

April 8th, 2016

April 8th, 2016

12,471 Views

1Division of Urologic Surgery, Washington University in St. Louis School of Medicine, 2Department of Urology, University of Wisconsin School of Medicine and Public Health, 3Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, 4O’Brien Urology Research Center, University of Wisconsin School of Medicine and Public Health

Immunohistochemistry is a powerful lab technique for evaluating protein localization and expression within tissues. Current semi-automated methods for quantitation introduce subjectivity and often create irreproducible results. Herein, we describe methods for multiplexed immunohistochemistry and objective quantitation of protein expression and co-localization using multispectral imaging.

Tags

Quantitation

-- Views

Related Videos

article

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid

article

Using the GELFREE 8100 Fractionation System for Molecular Weight-Based Fractionation with Liquid Phase Recovery

article

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

article

Quantitation of γH2AX Foci in Tissue Samples

article

Imaging Protein-protein Interactions in vivo

article

Design and Use of Multiplexed Chemostat Arrays

article

siRNA Screening to Identify Ubiquitin and Ubiquitin-like System Regulators of Biological Pathways in Cultured Mammalian Cells

article

Measurement of Metabolic Rate in Drosophila using Respirometry

article

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

article

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved