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Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

DOI :

10.3791/64758-v

8:18 min

April 7th, 2023

April 7th, 2023

767 Views

1Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center

Multiplexed barcoding image analysis has recently improved the characterization of the tumor microenvironment, permitting comprehensive studies of cell composition, functional state, and cell-cell interactions. Herein, we describe a staining and imaging protocol using the barcoding of oligonucleotide-conjugated antibodies and cycle imaging, which allows for the use of a high-dimensional image analysis technique.

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Multiplexed Barcoding Image Analysis

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