LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism

11.1K Views

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06:04 min

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April 4th, 2016

DOI :

10.3791/53941-v

April 4th, 2016

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Andrew J. Worth*¹^,², Dylan M. Marchione*²^,³, Robert C. Parry¹^,², Qingqing Wang²^,³, Kevin P. Gillespie²^,³, Noelle N. Saillant⁴, Carrie Sims⁴, Clementina Mesaros¹^,², Nathaniel W. Snyder⁵, Ian A. Blair¹^,²

¹Center for Cancer Pharmacology, University of Pennsylvania, ²Center for Excellence in Environmental Toxicology, University of Pennsylvania, ³Penn SRP and Department of Systems Pharmacology and Translational Therapeutics, University of Pennsylvania, ⁴Division of Traumatology, Department of Surgery, Critical Care and Acute Care Surgery, University of Pennsylvania, ⁵A.J. Drexel Autism Institute, Drexel University

Summary

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Keywords LC MS Analysis

Human Platelets

Mitochondrial Metabolism

Solid Phase Extraction

C18 Columns

Chapters in this video

0:05

Title

0:38

Preparation of Buffers and 100X Stock Solutions

1:35

Platelet Isolation and Performing an Experiment

2:49

Quenching and CoA Extraction

4:30

Results: Analysis of Human Platelets to Study Mitochondrial Metabolism

5:21

Conclusion

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