June 27th, 2016
•This video demonstrates a protocol to enrich thymic epithelial cells (TECs) with density gradient for FACS isolation. It also shows the use of EAK16-II/EAKIIH6 peptides to promote the TEC aggregate formation. The microenvironments of EAK16-II/EAKIIH6 hydrogel provide the 3-D configuration necessary to maintain the survival and function of the TECs.
Related Videos
Induction and Assessment of Class Switch Recombination in Purified Murine B Cells
In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells
Application of a Mouse Ligated Peyer’s Patch Intestinal Loop Assay to Evaluate Bacterial Uptake by M cells
Examination of Thymic Positive and Negative Selection by Flow Cytometry
Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells
Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus
Isolation, Identification, and Purification of Murine Thymic Epithelial Cells
Isolation and Transplantation of Different Aged Murine Thymic Grafts.
Preparation and Applications of Organotypic Thymic Slice Cultures
Analysis of Lymphocyte Extravasation Using an In Vitro Model of the Human Blood-brain Barrier
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved