Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions

Summary

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Keywords Polyvinyl Alcohol co itaconic Acid Hydrogels

Stem Cell Culture

Xeno free Conditions

Hydrogel Elasticity

Stem Cell Differentiation

Crosslinking

Hydrogel Preparation

P IA Films

Chapters in this video

0:04

Title

1:00

Polyvinyl Alcohol-Co-Itaconic Acid (P-IA) Hydrogel Dish Preparation and Grafting

5:02

Passaging of Undifferentiated Human Embryonic Stem (ES) and Human Induced Pluripotent Stem (iPS) Cells on Hydrogels

6:37

Characterization of the Expression of Pluripotency Proteins by Immunostaining

8:49

Results: Human ES and iPS Pluripotency on Conventional Dishes and Hydrogel Dishes

10:19

Conclusion

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