Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions
9.2K Views
•
11:37 min
•
February 3rd, 2018
DOI :
February 3rd, 2018
•Tzu-Cheng Sung*1, Hsing-Fen Li*1, Akon Higuchi1,2, Qing-Dong Ling3,4, Jia-Sin Yang1, Yeh-Chia Tseng1, Chih-Hsien Pan Pan1, Abdullah A. Alarfaj2, Murugan A. Munusamy2, Suresh Kumar5, Shih-Tien Hsu6, Kadarkarai Murugan7,8
1Department of Chemical and Materials Engineering, National Central University, 2Department of Botany and Microbiology, King Saud University, 3Cathay Medical Research Institute, Cathay General Hospital, 4Graduate Institute of Systems Biology and Bioinformatics, National Central University, 5Department of Medical Microbiology and Parasitology, Universiti Putra Malaysia, 6Department of Internal Medicine, Taiwan Landseed Hospital, 7Department of Zoology, Bharathiar University, 8Thiruvalluvar University
Chapters in this video
0:04
Title
1:00
Polyvinyl Alcohol-Co-Itaconic Acid (P-IA) Hydrogel Dish Preparation and Grafting
5:02
Passaging of Undifferentiated Human Embryonic Stem (ES) and Human Induced Pluripotent Stem (iPS) Cells on Hydrogels
6:37
Characterization of the Expression of Pluripotency Proteins by Immunostaining
8:49
Results: Human ES and iPS Pluripotency on Conventional Dishes and Hydrogel Dishes
10:19
Conclusion
Related Videos
07:33
A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media
7.8K Views
09:34
Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
9.2K Views
10:48
Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix
8.5K Views
10:50
Optimizing Attachment of Human Mesenchymal Stem Cells on Poly(ε-caprolactone) Electrospun Yarns
9.7K Views
07:45
Simple Polyacrylamide-based Multiwell Stiffness Assay for the Study of Stiffness-dependent Cell Responses
19.6K Views
10:54
Tunable Hydrogels from Pulmonary Extracellular Matrix for 3D Cell Culture
11.6K Views
10:04
Patterning the Geometry of Human Embryonic Stem Cell Colonies on Compliant Substrates to Control Tissue-Level Mechanics
8.1K Views
06:38
Comparative Study of Basement-Membrane Matrices for Human Stem Cell Maintenance and Intestinal Organoid Generation
1.1K Views
08:27
Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis
12.3K Views
08:20
Robust Tissue Fabrication for Long-Term Culture of iPSC-Derived Brain Organoids for Aging Research
3.2K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved