JoVE Logo
Faculty Resource Center

Sign In

A Simple Flow Cytometric Method to Measure Glucose Uptake and Glucose Transporter Expression for Monocyte Subpopulations in Whole Blood

DOI :

10.3791/54255-v

August 12th, 2016

August 12th, 2016

16,168 Views

1Centre for Biomedical Research, Macfarlane Burnet Institute for Medical Research and Public Health, 2Department of Infectious Diseases, Monash University, 3Department of Microbiology and Immunology, University of Melbourne, 4Department of Microbiology, The University of the West Indies, 5Division of Experimental Medicine, Department of Medicine, University of California, San Francisco, 6Department of Medicine, Monash University

Monocytes are integral components of the human innate immune system that rely on glycolytic metabolism when activated. We describe a flow cytometry protocol to measure glucose transporter expression and glucose uptake by total monocytes and monocyte subpopulations in fresh whole blood.

Tags

Flow Cytometry

-- Views

Related Videos

article

A Functional Whole Blood Assay to Measure Viability of Mycobacteria, using Reporter-Gene Tagged BCG or M.Tb (BCG lux/M.Tb lux)

article

A Simple and Efficient Method to Detect Nuclear Factor Activation in Human Neutrophils by Flow Cytometry

article

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

article

Measuring Granulocyte and Monocyte Phagocytosis and Oxidative Burst Activity in Human Blood

article

Flow Cytometric Analysis of Natural Killer Cell Lytic Activity in Human Whole Blood

article

Generation of Human Monocyte-derived Dendritic Cells from Whole Blood

article

Use of a Monocyte Monolayer Assay to Evaluate Fcγ Receptor-mediated Phagocytosis

article

A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines

article

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

article

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved