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Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols

DOI :

10.3791/54296-v

October 18th, 2016

October 18th, 2016

12,979 Views

1Department of Immunology, Herbert Wertheim College of Medicine, Florida International University, 2Millipore Sigma

This study compares two different methods of human monocyte isolation for obtaining in vitro dendritic cells (DCs). Monocytes are selected by adherence or negatively enriched by magnetic separation. Monocyte yield and viability along with MDDC viability, proliferation and CD11c/CD14 surface marker expression will be compared between both methods.

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