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A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model

DOI :

10.3791/54270-v

8:00 min

October 14th, 2016

October 14th, 2016

18,665 Views

1Institut pour la Recherche sur le Cancer de Lille (IRCL), INSERM, UMR-S-1172, Centre de Recherche Jean-Pierre Aubert (JPARC), 2Université de Lille

This manuscript provides a technical procedure that can be used to characterize C1498 cell cultures in vitro and the acute leukemia induced in mice after their injection. Phenotypic and functional analyses are performed using flow cytometry, immunofluorescence microscopy, cytochemistry and May-Grünwald Giemsa staining.

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Murine C1498 Cell Line

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