Preparation of Catheters and Uropathogenic E. coli for Infection
2:08
Transurethral Instillation of Female Mice
3:43
Transurethral Instillation of Male Mice
4:56
Determination of CFU in Infected Organs
6:09
Flow Cytometric Analysis of Bladder Tissue
8:31
Results: Transurethral Catheterization to Induce Urinary Tract Infections
9:57
Conclusion
Transcript
The overall goal of this experimental approach is to investigate the immune response to urinary tract infection, and to prevent direct comparison between male and female animals. This method can help answer key questions of mucosal immunology rela
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The established model of transurethral catheterization of mice allows the study of bladder pathologies, including urinary tract infection, but can only be performed in females. A new model of male transurethral instillation, presented here, will enable research in an area marked by strong clinical and epidemiological differences between the sexes.