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4D Multimodality Imaging of Citrobacter rodentium Infections in Mice

DOI :

10.3791/50450-v

12:38 min

August 13th, 2013

August 13th, 2013

12,592 Views

1MRC Centre for Molecular Bacteriology and Infection, Division of Cell & Molecular Biology, Imperial College London, 2Preclinical Imaging, Caliper- A PerkinElmer Company

Multi-modality imaging is a valuable approach for studying bacterial colonization in small animal models. This protocol outlines infection of mice with bioluminescent Citrobacter rodentium and the longitudinal monitoring of bacterial colonization using composite 3D diffuse light imaging tomography with μCT imaging to create a 4D movie of C. rodentium infection.

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4D Multimodality Imaging

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