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Rapid Subtractive Patterning of Live Cell Layers with a Microfluidic Probe

DOI :

10.3791/54447-v

12:19 min

September 15th, 2016

September 15th, 2016

6,666 Views

1IBM Research - Zurich

We present a protocol to perform subtractive patterning of live cell monolayers on a surface. This is achieved by local and selective lysis of adherent cells using a microfluidic probe (MFP). The cell lysate retrieved from local regions can be used for downstream analysis, enabling molecular profiling studies.

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Rapid Subtractive Patterning

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