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"Phagosome Closure Assay" to Visualize Phagosome Formation in Three Dimensions Using Total Internal Reflection Fluorescent Microscopy (TIRFM)

6.4K Views

10:07 min

August 26th, 2016

DOI :

10.3791/54470-v

August 26th, 2016


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Phagosome

Chapters in this video

0:05

Title

0:51

Red Blood Cell (RBC) Opsonization and Poly-lysine Coverslip Coating

2:31

Non-covalent SRBC Fixation

4:25

TIRF Microscopy Phagocytosis Visualization

5:23

Image Acquisition and Processing

7:45

Results: F-actin Accumulation at the Site of Phagosome Closure

8:51

Conclusion

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