JoVE Logo

Sign In

Measuring Phagosome pH by Ratiometric Fluorescence Microscopy

13.9K Views

14:39 min

December 7th, 2015

DOI :

10.3791/53402-v

December 7th, 2015


Explore More Videos

Keywords Phagosome

Chapters in this video

0:05

Title

1:02

Preparation of Phagocytic Targets

4:18

Live Video Microscopy

5:47

Calibration and Control Experiments

6:34

Analysis

11:44

Results: Time Courses, Average pH and Ratio Histograms of Wild-type and Hvcn1-/- Neutrophil Phagosomes

13:08

Conclusion

Related Videos

article

08:06

Study of Phagolysosome Biogenesis in Live Macrophages

14.3K Views

article

09:22

High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin Polymerization

14.6K Views

article

10:07

"Phagosome Closure Assay" to Visualize Phagosome Formation in Three Dimensions Using Total Internal Reflection Fluorescent Microscopy (TIRFM)

6.4K Views

article

12:42

Imaging the Neutrophil Phagosome and Cytoplasm Using a Ratiometric pH Indicator

10.0K Views

article

06:15

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy

12.5K Views

article

07:31

Measuring the pH, Redox Chemistries, and Degradative Capacity of Macropinosomes using Dual-Fluorophore Ratiometric Microscopy

2.3K Views

article

09:46

Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal Microscopy

14.9K Views

article

09:48

In vitro Quantitative Imaging Assay for Phagocytosis of Dead Neuroblastoma Cells by iPSC-Macrophages

5.4K Views

article

09:59

Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking

8.8K Views

article

02:47

TIRF Microscopy-Based Visualization of Phagosome Formation and Closure

40 Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved