December 7th, 2015
•Phagosomal pH influences phagosome maturation, oxidant production, phagosomal killing as well as antigen presentation. Here we describe a ratiometric method for measuring time-course and endpoint pH changes in individual phagosomes in living phagocytes using fluorescence microscopy.
Tags
Related Videos
Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy
Visualization of Bacterial Toxin Induced Responses Using Live Cell Fluorescence Microscopy
Study of Phagolysosome Biogenesis in Live Macrophages
Measuring Physiological Responses of Drosophila Sensory Neurons to Lipid Pheromones Using Live Calcium Imaging
Ratiometric Imaging of Extracellular pH in Dental Biofilms
"Phagosome Closure Assay" to Visualize Phagosome Formation in Three Dimensions Using Total Internal Reflection Fluorescent Microscopy (TIRFM)
Imaging the Neutrophil Phagosome and Cytoplasm Using a Ratiometric pH Indicator
Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation
Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
Measuring Deformability and Red Cell Heterogeneity in Blood by Ektacytometry
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved