Staphylococcus aureus Culturing and DNA Extraction
1:42
RS-PCR and Electrophoresis
4:19
Running the Analysis
5:32
Results: Staphylococcus aureus Genotype Identification by Ribosomal Spacer PCR
7:00
Conclusion
Transcript
The overall goal of this procedure is to obtain genotypes of Staphylococcus aureus, and to link them with disease in hosts. This method can help answer key questions in the field of bovine mastitis, to protect contagiousness and passaginicity of S
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Here, ribosomal spacer PCR (RS-PCR) is used together with a miniaturized electrophoresis system as a fast and high resolution method for genotyping S. aureus at moderate costs allowing a high throughput.