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DOI :
10.3791/54732-v
September 28th, 2016
Chapters
0:05
Title
1:08
Liposome Mediated Transient Transfection
2:35
Preparing Cell Samples for Staining
4:51
Extracellular Staining of Cell Samples
6:10
Intracellular Staining of Cell Samples
7:51
Data Analysis
8:55
Results: Simultaneaous Mutations of N-glycosylation Sites Disrupted Cell Surface Expression of CaVα2δ1
10:25
Conclusion
継承された不整脈は、しばしば、一つ以上のイオンチャネルの表面の配信を変更する突然変異によって引き起こされます。ここでは、TSA-201細胞で発現された組換えイオンチャネルの相対的な合計および細胞表面タンパク質発現の定量化を提供するために、フローサイトメトリーアッセイを適合させます。
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