Harvest of Transfected HEK293 Cells and Purification of rAAV9 Vectors
7:04
rAAV9 Injections into Neonatal Mice
8:10
Results: rAAV9 Overexpression and Gene Knockdown in Mouse Hearts
10:18
Conclusion
Transcript
The overall goal of this procedure is to prepare recombinant adeno-associated virus nine, or rAAV9, to manipulate gene expression in mouse hearts. The method can answer key question in the field of cardiology and molecular genetics, such as how to
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In this manuscript, a method to prepare recombinant adeno-associated virus 9 (rAAV9) vectors to manipulate gene expression in the mouse heart is described.