JoVE Logo

Sign In

Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression

9.3K Views

10:54 min

February 10th, 2017

DOI :

10.3791/54981-v

February 10th, 2017


Explore More Videos

RNA Sequencing

Chapters in this video

0:05

Title

0:59

Isolation of Embryonic Midbrain

2:55

Dissociation and Plating of Cells

5:34

Harvesting Cultured Dopaminergic Neurons for RNA Sequencing

7:04

Generation, Purification and Amplification of First-strand cDNA

9:13

Results: RNA-seq Detection of Expressed Transcripts in Single Dopaminergic Neurons

9:39

Conclusion

Related Videos

article

15:00

Intraventricular Transplantation of Engineered Neuronal Precursors for In Vivo Neuroarchitecture Studies

5.6K Views

article

07:30

Electrophysiological Characterization of GFP-Expressing Cell Populations in the Intact Retina

13.4K Views

article

09:17

Assessing Neurodegenerative Phenotypes in Drosophila Dopaminergic Neurons by Climbing Assays and Whole Brain Immunostaining

19.2K Views

article

08:02

An Enhanced Green Fluorescence Protein-based Assay for Studying Neurite Outgrowth in Primary Neurons

6.1K Views

article

08:25

Retrograde Tracing of Drosophila Embryonic Motor Neurons Using Lipophilic Fluorescent Dyes

6.1K Views

article

07:25

Identification of Dopamine D1-Alpha Receptor Within Rodent Nucleus Accumbens by an Innovative RNA In Situ Detection Technology

8.5K Views

article

09:36

Combined In Vivo Anatomical and Functional Tracing of Ventral Tegmental Area Glutamate Terminals in the Hippocampus

3.8K Views

article

13:13

Time-Lapse Imaging of Neuronal Arborization using Sparse Adeno-Associated Virus Labeling of Genetically Targeted Retinal Cell Populations

2.8K Views

article

04:17

Time-Lapse Imaging of Migrating Neurons and Glial Progenitors in Embryonic Mouse Brain Slices

794 Views

article

09:05

Multiplex Detection of Gene Expression in the Intact Drosophila Brain Using Expansion-Assisted Iterative Fluorescence In Situ Hybridization

485 Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved