In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

7.0K Views

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14:53 min

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February 3rd, 2018

DOI :

10.3791/55041-v

February 3rd, 2018

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Giovanna Muggiolu*¹^,², Marina Simon*¹^,², Nathanael Lampe¹^,², Guillaume Devès¹^,², Philippe Barberet¹^,², Claire Michelet¹^,², Marie-Hélène Delville³^,⁴, Hervé Seznec¹^,²

¹Centre d'Etudes Nucléaires Bordeaux Gradignan (CENBG), Université de Bordeaux, ²Centre d'Etudes Nucléaires Bordeaux Gradignan (CENBG), CNRS, ³Institut de Chimie de la Matière Condens é e de Bordeaux (ICMCB), CNRS, ⁴Institut de Chimie de la Matière Condens é e de Bordeaux (ICMCB), Université de Bordeaux

Summary

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Keywords Nanoparticles

Nuclear Microprobe Analysis

Single Cell Quantification

In Situ Detection

Fluorescence Microscopy

Chapters in this video

0:00

Title

1:38

Sample Holder Preparation

3:38

Nanoparticles Preparation and Exposure

5:05

Paraformaldehyde fixation for Fluorescence Microscopy

5:26

Plunge Freezing Fixation and Dehydration

7:13

Nuclear Microprobe Analysis

8:58

Data Analysis

10:56

Representative results

13:12

Conclusion

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