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JoVE Journal

Chemistry

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In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

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14:53 min

February 3rd, 2018

February 3rd, 2018

7,003 Views

0:00

Title

1:38

Sample Holder Preparation

3:38

Nanoparticles Preparation and Exposure

5:05

Paraformaldehyde fixation for Fluorescence Microscopy

5:26

Plunge Freezing Fixation and Dehydration

7:13

Nuclear Microprobe Analysis

8:58

Data Analysis

10:56

Representative results

13:12

Conclusion

Transcript

Nanoparticles are increasingly used in industry and medicine due to their unique physiochemical properties. However, concerns for human health associated with prolonged nanoparticle exposure persists. These risks can be quantified by studying the

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We describe a procedure for the detection of chemical elements present in situ in human cells as well as their in vitro quantification. The method is well-suited to any cell type and is particularly useful for quantitative chemical analyses in single cells following in vitro metal oxide nanoparticles exposure.

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