The WinCF Model - An Inexpensive and Tractable Microcosm of a Mucus Plugged Bronchiole to Study the Microbiology of Lung Infections

9.0K Views

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06:57 min

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May 8th, 2017

DOI :

10.3791/55532-v

May 8th, 2017

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William J. Comstock¹, Edwin Huh², Reiley Weekes², Connor Watson², Tianyang Xu², Pieter C. Dorrestein¹, Robert A. Quinn¹

¹Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, ²Department of Mechanical and Aerospace Engineering, University of California, San Diego

Summary

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Keywords WinCF Model

Artificial Sputum Medium

Mucus Plugged Bronchiole

Chapters in this video

0:05

Title

0:38

Preparing Medium and Artificial Sputum for Capillary Tube Loading

2:29

Filling the WinCF Capillary Tubes with Inoculated Artificial Sputum Medium

4:12

Incubation and Imaging the Extraction

5:38

Results: Microbiological Growth in the WinCF Capillary Tubes

6:22

Conclusion

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