P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives
10.7K Views
•
10:26 min
•
June 15th, 2020
DOI :
June 15th, 2020
•Carlos Victor Montefusco-Pereira*1,2, Justus C. Horstmann*1,2, Thomas Ebensen3, Christoph Beisswenger4, Robert Bals4, Carlos A. Guzmán3, Nicole Schneider-Daum1, Cristiane de Souza Carvalho-Wodarz1, Claus-Michael Lehr1,2
1Department of Drug Delivery, Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), 2Department of Pharmacy, Saarland University, 3Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, 4Department of Internal Medicine V - Pulmonology, Allergology, Respiratory Intensive Care Medicine, Saarland University Hospital
Explore More Videos
Chapters in this video
0:04
Introduction
1:26
Growth and Differentiation of Cells in Permeable Support Inserts
5:29
Infection by P. aeruginosa
7:00
Measurement of Bacterial Proliferation via Colony-forming Units (CFU)
8:00
Results: Epithelial-macrophage Co-cultures Infected with P. aeruginosa
9:48
Conclusion
Related Videos
11:21
Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells
22.1K Views
07:46
Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung
32.8K Views
12:09
Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro
22.4K Views
15:43
Long Term Chronic Pseudomonas aeruginosa Airway Infection in Mice
22.6K Views
06:43
Studying Microbial Communities In Vivo: A Model of Host-mediated Interaction Between Candida Albicans and Pseudomonas Aeruginosa in the Airways
8.5K Views
07:25
Visualization of Pseudomonas aeruginosa within the Sputum of Cystic Fibrosis Patients
3.0K Views
09:26
Antibiotic Efficacy Testing in an Ex vivo Model of Pseudomonas aeruginosa and Staphylococcus aureus Biofilms in the Cystic Fibrosis Lung
6.8K Views
07:39
Tools for the Real-Time Assessment of a Pseudomonas aeruginosa Infection Model
3.3K Views
07:16
Development of a Polymicrobial Colony Biofilm Model to Test Antimicrobials in Cystic Fibrosis
832 Views
09:29
An Air-liquid Interface Bronchial Epithelial Model for Realistic, Repeated Inhalation Exposure to Airborne Particles for Toxicity Testing
11.4K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved